Dembele joined knee injury victim Victor Wanyama on the sidelines along with Eric Dier, who is nursing a thigh problem, to leave young England prospect Harry Winks as the only specialist central midfielder at Pochettino's disposal.
The 22-year-old was left out of Tottenham's squad that registered a 3-2 win over Wolves at Molineux on Saturday as Mauricio Pochettino opted to manage his squad carefully ahead of a Champions League meeting with PSV Eindhoven this week.
"With the 3-0 lead I think everyone thought the game was over, but when you make a mistake you need to play more seriously and not concede so cheaply", the Spurs boss told Sky Sports.
Wolves slipped to their third straight defeat despite their late rally but defender Ryan Bennett insisted they will solve any issues.
The South Korean played a one-two with Lamela, who brought down the return pass on his chest and poked a right-footed finish through the legs of Rui Patricio for his fourth goal in his last five Premier League starts.
Although, fans were finally given something to cheer about on Wednesday night as Tottenham ran out 3-1 winners against West Ham at the London Stadium in the Carabao Cup.
He was replaced from the substitutes' bench by Son Heung-Min in a change that forced a reshuffle, with Moussa Sissoko moving to his less favoured holding midfield role.More news: Football leaks exposes Premier League clubs at heart of planned European Superleague
DF Kieran Trippier, 5 - Delivered a fine half-volleyed cross for Lucas' goal, but cancelled that out at the other end when his attempt to nutmeg an opponent in a unsafe area led to Wolves' first penalty. A wrongly disallowed goal left them feeling hard done by.
The hosts were awarded a second penalty in the 77th minute as Juan Foyth committed another foul in the box. "But the most important thing is we got the three points".
DF Ben Davies, 5 - Struggled against Matt Doherty throughout and, once again, offered precious little in possession.
It promises to be a fascinating encounter as Wolves look to get back to winning ways against Tottenham Hotspur.
MF Mousa Dembele, N/R - Suffered an ankle injury inside the opening two minutes, ending his involvement.
"He had good moments in part of the game [in the league against Spurs], he also had bad moments, but he is not the first player that needs some time to know what is the Premier League".
62- Goal! Harry Kane lashes it home from close range! After 79, it was 3-2 because of two penalties conceded through carelessness.